I011-1539, I011-1582, I011-1605, I011-1662, I011-1680 & I011-1704
For a number of years Cargille Laboratories has been using the Meltmount Quick-Stick for the preparation of Cargille-Allen reference set slides.
- Do all work in a well ventilated area
- Meltmount is a thermoplastic: it is fluid when heated and functionally a solid at room temperature; the appearance of the prepared slide will remain unchanged after the slide is returned to room temperature.
- The most common problem encountered is: the inclusion of bubbles in the mount. The procedure described here should reduce or eliminate this problem.
- Meltmount Quick-Stick is conveniently enclosed in a Teflon® tube that can be cut back to expose the Meltmount so that it can be applied directly to a heated slide, making slide preparation quick and neat.
- Meltmount is a Thermal Plastic Material. This means its viscosity is dependent on temperature (inversely dependent). As the temperature increases the viscosity decreases. There is no sharp melting point. Being thermal plastic, it is capable of "cold flow". This means the Meltmount, the specimen, the slide, and the cover slip can all move independently of each other given a mix of time, temperature, and lateral pressure or gravity.
Storage of prepared slide: treat them as the valuable items they are. Store:
- Flat, cover slip on top
- In the dark
- Away from dust and fumes
- At 15° to 30°C. Meltmount is meant to be thermally reversible, don't allow this to happen inadvertently by storing or transporting prepared slide above 29°C.
The Procedure for Using Meltmount™ Quick-Stick™
- Adjust hot plate for medium heat (ideally 60°C to 70°C).
- Remove the tall cap bearing the label.
- When using Meltmount Quick-Stick I011-1582, I011-1662, I011-1680 and I011-1704 (but not when using I011-1539), firmly roll the end to be used between your thumb and index finger or on a hard surface such as a lab table top; this will break the adhesion of the Meltmount to the Teflon tube.
- Use a single edged razor blade to cut off and remove the Teflon to expose ¼ to ½ inch of Meltmount.
- Put a trial slide on the hot plate and apply Meltmount to its center. The melted Meltmount on the slide should be thin and watery but not smoking. If not, adjust the temperature up to make thinner or down to avoid smoking (60°C to 70°C is ideal).
- Put a slide on the hot plate with about ¼ of the slide off the hot plate's surface where it will remain cool so it can be handled.
- Use the Quick-Stick to apply a 2cm patch of Meltmount to the centre of the slide.
- Put the specimen in the centre of the patch of the Meltmount, you may want to remove the slide from the hot plate to do this, and then return it to the hot plate.
- Do the following as quickly as possible
- Place the cover glass completely on the hot plate surface. Apply a 2cm size patch of Meltmount to the centre of the coverglass.
- Raise the Quick-Stick from the hot plate WITH the cover glass attached to the Quick-Stick.
- Remove the cover glass from the Quick-Stick, invert and drop (Meltmount side down) onto the centre of the slide.
- Use a pencil eraser to centre cover glass while pressing to remove bubbles.
- Remove mounted slide from the hot plate.
Note: Heat Sensitive Specimens may be altered by this method, in which case, mounts can be made without heat by The Pressure Method described later in these instructions.
- If the area beneath the cover glass is not completely filled with Meltmount, return the slide to the hot plate and ass more Meltmount by touching the Quick-Stick to the edge of the cover glass. Then remove from the heat.
- Optional: The cleaning of the mounted slide and removal of excess Meltmount can be done as follows:When the slide has cooled to room temperature, scrape of excess Meltmount using as a tool a singleedge razor blade or dental scraping tool. The tool can be dipped frequently in cold water to keep it cook and avoid sticking.
- Soak the slide in a tray of Windex® window cleaner.
- While immersed, clean around the edge of the cover glass using a sponge tipped swab (a cotton tipped swab will leave fibres behind.
- Remove the slide from the tray and do the final cleaning with a lint free tissue.
The Pressure Method
Note: This method is useful for making permanent slides in the field, or for making permanent slides of heat sensitive specimens.
- Turn on hot plate to medium heat (ideally 60°C to 70°C).
- Place clean slide on hot plate with ¼ of the slide off of the hot plate so that this portion will remain cool so that you can pick it up with your fingers.
- With the Quick-Stick apply a patch of Meltmount that is the size of a coverglass.
- Remove slide from hot plate and cool to room temperature.
- Store slides with Meltmount in a slide box, with slides held flat.
- Applying the specimen can be done in many ways such as:
- Drop specimen onto the patch of Meltmount
- Place the Meltmount patch on the slide directly against the specimen.
- Transfer specimen to sticky tape, then transfer from tape to the Meltmount patch on the slide by running your fingernail over the back of the tape.
- The cover glass can be used either with or without first applying to it a layer of Meltmount. The Meltmount can be applied to one side of the cover glass as it sits on the hot plate. When the cover glass has been removed and has cooled it can be placed over a the patch of Meltmount containing the specimen on the slide. In the field it may be more convenient to place a cover glass WITHOUT Meltmount on top of the Meltmount patch containing the specimen on the slide.
- Press the cover glass and slide together using thumb and forefinger, taking care not to crack the cover glass.
- If desires, the finished slide can be improved by one of the following methods:
- Apply pressure of thumb and forefinger for a longer time.
- Heat briefly on a hot plate.
- Place a waxed paper covered weight, such as a book, on top of the mounted slide overnight.