SAFETY DATA SHEET
Gram's crystal violet solution
1. IDENTIFICATION
Product Identifiers
Product Name:
Gram's crystal violet solution
Other Names:
No Data
Product Number(s):
AGK - GRAM'S CRYSTAL VIOLET SOLUTION
CAS Number:
No data
Recommended use of the chemical and restriction on use
Laboratory use. Laboratory stain/reagent. Staining or micro-organisms.
Company Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
Emergency Contact Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
2. HAZARDS IDENTIFICATION
Classification of the substance or mixture
Flammable liquids (Category 2), H225
Serious eye damage/eye irritation (Category 2), H319
Carcinogenicity (Category 2), H351
Specific target organ toxicity - single exposure (Category 3), Central nervous system, H336
Long-term (chronic) aquatic hazard (Category 2), H411
Serious eye damage/eye irritation (Category 2), H319
Carcinogenicity (Category 2), H351
Specific target organ toxicity - single exposure (Category 3), Central nervous system, H336
Long-term (chronic) aquatic hazard (Category 2), H411
Label Elements
Signal Words
Danger
Hazard Statement(s)
H225 Highly flammable liquid and vapour.
H319 Causes serious eye irritation.
H336 May cause drowsiness or dizziness.
H351 Suspected of causing cancer.
H411 Toxic to aquatic life with long lasting effects.
H319 Causes serious eye irritation.
H336 May cause drowsiness or dizziness.
H351 Suspected of causing cancer.
H411 Toxic to aquatic life with long lasting effects.
Precautionary Statement(s)
P201 Obtain special instructions before use.
P210 Keep away from heat/ sparks/ open flames/ hot surfaces. No smoking.
P233 Keep container tightly closed.
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P273 Avoid release to the environment.
P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.
P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P304 + P340 + P312 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/ doctor if you feel unwell.
P370 + P378 In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P391 Collect spillage.
P210 Keep away from heat/ sparks/ open flames/ hot surfaces. No smoking.
P233 Keep container tightly closed.
P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P273 Avoid release to the environment.
P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.
P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P304 + P340 + P312 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/ doctor if you feel unwell.
P370 + P378 In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P391 Collect spillage.
Primary route(s) of entry
Inhalation (skin); Absorbtion (skin)
Human Health
Inhalation:
Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
May cause drowsiness or dizziness.
May cause drowsiness or dizziness.
Ingestion:
No Data
Eyes:
Causes serious eye irritation
Skin:
Take off immediately all contaminated clothing. Rinse skin with water/ shower.
Environment
Toxic to aquatic life with long lasting effects. Dispose of contents/container in accordance with local regional/national/international regulations.
3. COMPOSITION/INFORMATION ON INGREDIENTS
Name
CAS No.
Content (w/w)
Classification
Ethanol
64-17-5
>= 1 - < 10 %
Flam. Liq. 2; 2A; H225, H319 Concentration limits: >= 50 %: Eye Irrit. 2A
Crystal violet
548-62-9
>= 0.25 - < 1 %
Acute Tox. 4; 1; Carc. 2; Aquatic Acute 1; Aquatic Chronic 1; H302, H318, H351, H400, H410 M-Factor - Aquatic Acute: 10 M-Factor - Aquatic Chronic: 10
4. FIRST AID MEASURES
Ingestion
After swallowing: make victim drink water (two glasses at most). Consult doctor if feeling unwell.
Inhalation
After inhalation: fresh air.
Skin Contact
In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with water/ shower.
Eye Contact
After eye contact: rinse out with plenty of water. Remove contact lenses.
Other Information
No Data
5. FIREFIGHTING MEASURES
Suitable extinguishing equipment
Water Foam Carbon dioxide (CO₂) Dry powder
HAZCHEM
Special protective equipment and precautions for fire fighters
6. ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency procedures
Advice for non-emergency personnel: Do not breathe vapours, aerosols. Ensure adequate ventilation. Keep away from heat and sources of ignition. Evacuate the danger area, observe emergency procedures, consult an expert.
Environmental precautions
Do not let product enter drains. Risk of explosion.
Methods and materials for containment and clean up
Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions. Take up with liquid-absorbent material (e.g. Chemizorb®). Dispose of properly. Clean up affected area.
7. HANDLING AND STORAGE
Precautions for safe handling
Keep away from open flames, hot surfaces and sources of ignition.Take precautionary measures against static discharge. Change contaminated clothing. Wash hands after working with substance.
Conditions for safe storage
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Exposure Standards
Material
TWA ppm
TWA mg/m3
STEL ppm
STEL mg/m3
Ethanol 64-17-5
1,000 ppm
1,880 mg/m3
-
Engineering controls
Change contaminated clothing. Wash hands after working with substance.
Personal protective equipment
Eye and face protection
Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Safety glasses
Skin protection
Full contact
Material: butyl-rubber
Minimum layer thickness: 0.7 mm
Break through time: 480 min
Material tested:Butoject® (KCL 898)
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0.40 mm
Break through time: 120 min
Material tested:Camatril® (KCL 730 / Aldrich Z677442, Size M)
Material: butyl-rubber
Minimum layer thickness: 0.7 mm
Break through time: 480 min
Material tested:Butoject® (KCL 898)
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0.40 mm
Break through time: 120 min
Material tested:Camatril® (KCL 730 / Aldrich Z677442, Size M)
Body protection
Flame retardant antistatic protective clothing.
Respiratory protection
Required when vapours/aerosols are generated.
9. PHYSICAL AND CHEMICAL PROPERTIES
General information
Appearance
Liquid, blue
Odour
phenol-like
pH
No data available
Vapour Pressure
No data available
Density
0.99 g/cm3 at 20 °C
Boiling Point
No data available
Melting Point
No data available
Solubility
at 20 °C soluble
Specific Gravity of Density
No data available
Flash Point
47 °C
Flammable (Explosive) Limits
No data available
Ignition Temperature
No data available
Formula
10. STABILITY AND REACTIVITY
Reactivity
Vapor/air-mixtures are explosive at intense warming.
Chemical stability
The product is chemically stable under standard ambient conditions (room temperature)
Possibility of hazardous reactions
Violent reactions possible with: The generally known reaction partners of water.
Conditions to avoid
Heating
Incompatible materials
No data available
11. TOXICOLOGICAL INFORMATION
Acute effects
Acute toxicity estimate Oral - > 2,000 mg/kg (Calculation method)
Acute toxicity estimate Inhalation - 4 h - > 5 mg/l - dust/mist(Calculation method)
Acute toxicity estimate Dermal - > 2,000 mg/kg (Calculation method)
Acute toxicity estimate Inhalation - 4 h - > 5 mg/l - dust/mist(Calculation method)
Acute toxicity estimate Dermal - > 2,000 mg/kg (Calculation method)
Eye contact
No data available
Skin contact
No data available
Ingestion
No data available
Inhalation
No data available
Toxicity and irritation
ETHANOL
LD50 Oral - Rat - male and female - 10,470 mg/kg (OECD Test Guideline 401)
LC50 Inhalation - Rat - male and female - 4 h - 124.7 mg/l - vapour (OECD Test Guideline 403)
Dermal: No data available
Skin corrosion/irritation
Skin - Rabbit
Result: No skin irritation - 24 h (OECD Test Guideline 404)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye irritation. (OECD Test Guideline 405)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye irritation. (OECD Test Guideline 405)
Germ cell mutagenicity
Test Type: Ames test
Test system: Salmonella typhimurium
Result: negative
Test Type: In vitro mammalian cell gene mutation test
Test system: mouse lymphoma cells
Result: negative
Method: OECD Test Guideline 478
Species: Mouse - male
Result: Positive results were obtained in some in vivo tests.
CRYSTAL VIOLET
Acute toxicity
LD50 Oral - Rat - 420 mg/kg
Remarks: (RTECS)
Inhalation: No data available
Dermal: No data available
Skin corrosion/irritation
Skin - Rabbit
Result: No skin irritation - 24 h Remarks: (ECHA)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye damage.
Remarks: (HSDB)
Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2)
Respiratory or skin sensitization - No data available
Germ cell mutagenicity
Test Type: In vitro mammalian cell gene mutation test
Test system: Chinese hamster ovary cells
Result: negative
Remarks: (ECHA)
Test Type: Ames test
Test system: Salmonella typhimurium
Result: negative
Remarks: (National Toxicology Program)
Carcinogenicity - Suspected of causing cancer.
Reproductive toxicity - No data available
Specific target organ toxicity - single exposure - No data available
Specific target organ toxicity - repeated exposure
Aspiration hazard - No data available
LD50 Oral - Rat - male and female - 10,470 mg/kg (OECD Test Guideline 401)
LC50 Inhalation - Rat - male and female - 4 h - 124.7 mg/l - vapour (OECD Test Guideline 403)
Dermal: No data available
Skin corrosion/irritation
Skin - Rabbit
Result: No skin irritation - 24 h (OECD Test Guideline 404)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye irritation. (OECD Test Guideline 405)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye irritation. (OECD Test Guideline 405)
Germ cell mutagenicity
Test Type: Ames test
Test system: Salmonella typhimurium
Result: negative
Test Type: In vitro mammalian cell gene mutation test
Test system: mouse lymphoma cells
Result: negative
Method: OECD Test Guideline 478
Species: Mouse - male
Result: Positive results were obtained in some in vivo tests.
CRYSTAL VIOLET
Acute toxicity
LD50 Oral - Rat - 420 mg/kg
Remarks: (RTECS)
Inhalation: No data available
Dermal: No data available
Skin corrosion/irritation
Skin - Rabbit
Result: No skin irritation - 24 h Remarks: (ECHA)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: Causes serious eye damage.
Remarks: (HSDB)
Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2)
Respiratory or skin sensitization - No data available
Germ cell mutagenicity
Test Type: In vitro mammalian cell gene mutation test
Test system: Chinese hamster ovary cells
Result: negative
Remarks: (ECHA)
Test Type: Ames test
Test system: Salmonella typhimurium
Result: negative
Remarks: (National Toxicology Program)
Carcinogenicity - Suspected of causing cancer.
Reproductive toxicity - No data available
Specific target organ toxicity - single exposure - No data available
Specific target organ toxicity - repeated exposure
Aspiration hazard - No data available
12. ECOLOGICAL INFORMATION
Ecotoxicity
No data available
Persistence and degradability
No data available
Bioaccumulative potential
No data available
Other adverse effects
Ethanol
Toxicity to fish flow-through test LC50 - Pimephales promelas (fathead minnow) - 15,300 mg/l - 96 h (US-EPA)
Toxicity to daphnia and other aquatic invertebrates
static test LC50 - Ceriodaphnia dubia (water flea) - 5,012 mg/l - 48 h Remarks: (ECHA)
Toxicity to algae static test ErC50 - Chlorella vulgaris (Fresh water algae) - 275mg/l - 72 h (OECD Test Guideline 201)
Toxicity to bacteria static test IC50 - activated sludge - > 1,000 mg/l - 3 h (OECD Test Guideline 209)
Crystal violet
Toxicity to fish static test LC50 - Pimephales promelas (fathead minnow) - 0.082 mg/l - 96 h Remarks: (ECHA)
Toxicity to daphnia and other aquatic invertebrates static test EC50 - Daphnia magna (Water flea) - > 0.24 - < 0.5 mg/l - 48 h (OECD Test Guideline 202)
Toxicity to algae static test ErC50 - Pseudokirchneriella subcapitata (green algae) - > 0.025 - < 0.8 mg/l - 72 h (OECD Test Guideline 201)
Toxicity to bacteria EC50 - Bacteria - 10 - 100 mg/l Remarks: (External MSDS)
Toxicity to fish flow-through test LC50 - Pimephales promelas (fathead minnow) - 15,300 mg/l - 96 h (US-EPA)
Toxicity to daphnia and other aquatic invertebrates
static test LC50 - Ceriodaphnia dubia (water flea) - 5,012 mg/l - 48 h Remarks: (ECHA)
Toxicity to algae static test ErC50 - Chlorella vulgaris (Fresh water algae) - 275mg/l - 72 h (OECD Test Guideline 201)
Toxicity to bacteria static test IC50 - activated sludge - > 1,000 mg/l - 3 h (OECD Test Guideline 209)
Crystal violet
Toxicity to fish static test LC50 - Pimephales promelas (fathead minnow) - 0.082 mg/l - 96 h Remarks: (ECHA)
Toxicity to daphnia and other aquatic invertebrates static test EC50 - Daphnia magna (Water flea) - > 0.24 - < 0.5 mg/l - 48 h (OECD Test Guideline 202)
Toxicity to algae static test ErC50 - Pseudokirchneriella subcapitata (green algae) - > 0.025 - < 0.8 mg/l - 72 h (OECD Test Guideline 201)
Toxicity to bacteria EC50 - Bacteria - 10 - 100 mg/l Remarks: (External MSDS)
13. DISPOSAL CONSIDERATIONS
General information
Waste material must be disposed of in accordance with the national and local regulations. Leave chemicals in original containers. No mixing with other waste. Handle uncleaned containers like the product itself. See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact us there if you have further questions.
14. TRANSPORT INFORMATION
ADG label required
HAZCHEM
UN Number
UN1170
Proper shipping name
Ethanol solution
Transport hazard class
3
Packing group
III
Environmental hazard
No data
Special precautions for users
No data
Additional information
No data
15. REGULATORY INFORMATION
Poisons Schedule Number
2Y
Other Information
No Data
16. OTHER INFORMATION
SDS preparation date
8 March 2023
Comments
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship.This Safety Data Sheet (SDS) has been prepared in compliance with the Preparation of Safety Data Sheets for Hazardous Chemicals Code of Practice February 2016. It is the user's responsibility to determine the suitability of this information for adoption of necessary safety precautions. The information published in this SDS has been compiled from the publications listed in Section 16: to the best of our ability and knowledge these publications are considered accurate. We reserve the right to revise Safety Data Sheets as new information becomes available. Copies may be made for non-profit use.
This Safety Data Sheet (SDS) has been prepared in compliance with the Preparation of Safety Data Sheets for Hazardous Chemicals Code of Practice February 2016. It is the user's responsibility to determine the suitability of this information for adoption of necessary safety precautions. The information published in this SDS has been compiled from the publications listed in Section 16: to the best of our ability and knowledge these publications are considered accurate. We reserve the right to revise Safety Data Sheets as new information becomes available. Copies may be made for non-profit use.