SAFETY DATA SHEET
Indigo Carmine, C.I. 73015
1. IDENTIFICATION
Product Identifiers
Product Name:
Indigo Carmine, C.I. 73015
Other Names:
Acid Blue 74; C.I. 73015; Indigo-5,5'-Indigodisulfonic acid, disodium salt.
Product Number(s):
C111, C1111, C1115
CAS Number:
860-22-0
Recommended use of the chemical and restriction on use
Laboratory use. Stain for Electron Microscopy.
Company Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
Emergency Contact Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
11 Carlton Street
KIRWAN QLD 4817
Australia(07) 4773 9444www.proscitech.com
2. HAZARDS IDENTIFICATION
Classification of the substance or mixture
Acute toxicity, Oral (Category 4)
Label Elements
Signal Words
Warning
Hazard Statement(s)
H302: Harmful if swallowed.
H317 May cause an allergic skin reaction.
H317 May cause an allergic skin reaction.
Precautionary Statement(s)
wash skin thoroughly after handling.
Do not drink, eat, smoke, when using this product. Rinse Mouth.
If Swallowed: Call Poison Center or doctor/physician if you feel unwell.
Dispose of contents/container to an approved waste disposal plant.
Do not drink, eat, smoke, when using this product. Rinse Mouth.
If Swallowed: Call Poison Center or doctor/physician if you feel unwell.
Dispose of contents/container to an approved waste disposal plant.
Primary route(s) of entry
Primary route: Inhilation
Scondary route: Skin contact, Eye contact.
Scondary route: Skin contact, Eye contact.
Human Health
Inhalation:
Harmful by inhalation.
Ingestion:
Harmful if swallowed.
Eyes:
Risk of serious damage to eyes.
Skin:
Harmful in contact with skin.
Environment
Not available.
3. COMPOSITION/INFORMATION ON INGREDIENTS
Name
CAS No.
Content (w/w)
Classification
Disodium 5,5'(2-(1,3-dihydro-3-oxo-2H-indazol-2-ylidene)-1,2-dihydro- 3H-indol-3Disodium 5,5'(2-(1,
860-22-0
100%
4. FIRST AID MEASURES
Ingestion
Do not induce vomiting without medical advice. Rinse with water. Consult physician.
Inhalation
Move to fresh air. Consult a physician.
Skin Contact
Rinse with plenty of soap & water and seek medical advice.
Eye Contact
Rinse opened eye for several minutes under running water and seek medical advice.
Other Information
Treat symptomatically and supportively.
5. FIREFIGHTING MEASURES
Suitable extinguishing equipment
In case of fire, use water, dry chemical, chemical foam, or alcohol-resistant foam.
HAZCHEM
Carbon, sulphur, sodium & nitrogen oxides
Special protective equipment and precautions for fire fighters
6. ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency procedures
Use proper personal protective equipment as indicated in Section 8.
Environmental precautions
Not available.
Methods and materials for containment and clean up
Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Provide ventilation.
7. HANDLING AND STORAGE
Precautions for safe handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation. Store protected from light.
Conditions for safe storage
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Exposure Standards
Engineering controls
Facilities storing or utilising this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Personal protective equipment
Eye and face protection
Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin protection
Protection of hands:
The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.
Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ the chemical mixture.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation.
Material of gloves
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer.
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.
Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ the chemical mixture.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation.
Material of gloves
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer.
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
Body protection
Wear appropriate protective clothing to prevent skin exposure.
Respiratory protection
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.
9. PHYSICAL AND CHEMICAL PROPERTIES
General information
Appearance
Dark blue to dark purple powder.
Odour
Not available.
pH
Not available.
Vapour Pressure
Not available.
Density
0.71 g/cm3 (29 °C)
Boiling Point
Not available.
Melting Point
340 °C
Solubility
1 g/l (27 °C)
Specific Gravity of Density
Not available.
Flash Point
Not applicable.
Flammable (Explosive) Limits
Not available.
Ignition Temperature
Not applicable.
Formula
C15H15N3.HCl
10. STABILITY AND REACTIVITY
Reactivity
No further relevant information available.
Chemical stability
Stable under ordinary conditions of use and storage. Light sensitive.
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Possibility of hazardous reactions
No further relevant information available.
Conditions to avoid
Incompatible materials, light.
Incompatible materials
No data available.
11. TOXICOLOGICAL INFORMATION
Acute effects
No further relevant information available.
Eye contact
No further relevant information available.
Skin contact
No further relevant information available.
Ingestion
No further relevant information available.
Inhalation
No further relevant information available.
Toxicity and irritation
LD50/LC50: Oral, rat: LD50 = 2 gm/kg;
Carcinogenic categories
· IARC (International Agency for Research on Cancer)
Substance is not listed.
· NTP (National Toxicology Program)
Substance is not listed.
· OSHA-Ca (Occupational Safety Health Administration)
Substance is not listed.
Carcinogenic categories
· IARC (International Agency for Research on Cancer)
Substance is not listed.
· NTP (National Toxicology Program)
Substance is not listed.
· OSHA-Ca (Occupational Safety Health Administration)
Substance is not listed.
12. ECOLOGICAL INFORMATION
Ecotoxicity
No further relevant information available.
Persistence and degradability
No further relevant information available.
Bioaccumulative potential
No further relevant information available.
Other adverse effects
Water hazard class 1 (Assessment by list): slightly hazardous for water.
13. DISPOSAL CONSIDERATIONS
General information
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. RCRA P-Series: None listed. RCRA U-Series: None listed.
14. TRANSPORT INFORMATION
ADG label required
HAZCHEM
Carbon, sulphur, sodium & nitrogen oxides
UN Number
None
Proper shipping name
Not regulated.
Transport hazard class
Void
Packing group
None.
Environmental hazard
Not controlled.
Special precautions for users
Not regulated.
Additional information
No information available.
15. REGULATORY INFORMATION
Poisons Schedule Number
None Allocated.
Other Information
Sara
· Section 355 (extremely hazardous substances): Substance is not listed.
· Section 313 (Specific toxic chemical listings): Substance is not listed.
· TSCA (Toxic Substances Control Act): ACTIVE
Hazardous Air Pollutants
Substance is not listed.
· Proposition 65
· Chemicals known to cause cancer: Substance is not listed.
· Chemicals known to cause reproductive toxicity for females: Substance is not listed.
· Chemicals known to cause reproductive toxicity for males: Substance is not listed.
· Chemicals known to cause developmental toxicity: Substance is not listed.
Carcinogenic categories
· EPA (Environmental Protection Agency): Substance is not listed.
· TLV (Threshold Limit Value): Substance is not listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health): Substance is not listed.
· Section 355 (extremely hazardous substances): Substance is not listed.
· Section 313 (Specific toxic chemical listings): Substance is not listed.
· TSCA (Toxic Substances Control Act): ACTIVE
Hazardous Air Pollutants
Substance is not listed.
· Proposition 65
· Chemicals known to cause cancer: Substance is not listed.
· Chemicals known to cause reproductive toxicity for females: Substance is not listed.
· Chemicals known to cause reproductive toxicity for males: Substance is not listed.
· Chemicals known to cause developmental toxicity: Substance is not listed.
Carcinogenic categories
· EPA (Environmental Protection Agency): Substance is not listed.
· TLV (Threshold Limit Value): Substance is not listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health): Substance is not listed.
16. OTHER INFORMATION
SDS preparation date
23 April 2026
Comments
Abbreviations and acronyms:
ADR: Accord relatif au transport international des marchandises dangereuses par route (European Agreement Concerning the International
Carriage of Dangerous Goods by Road)
IMDG: International Maritime Code for Dangerous Goods
DOT: US Department of Transportation
IATA: International Air Transport Association
EINECS: European Inventory of Existing Commercial Chemical Substances
CAS: Chemical Abstracts Service (division of the American Chemical Society)
NFPA: National Fire Protection Association (USA)
HMIS: Hazardous Materials Identification System (USA)
VOC: Volatile Organic Compounds (USA, EU)
LC50: Lethal concentration, 50 percent
LD50: Lethal dose, 50 percent
PBT: Persistent, Bioaccumulative and Toxic
vPvB: very Persistent and very Bioaccumulative
NIOSH: National Institute for Occupational Safety
OSHA: Occupational Safety Health
TLV: Threshold Limit Value
PEL: Permissible Exposure Limit
REL: Recommended Exposure Limit
Acute toxicity - oral 4: Acute toxicity - Category 4
ADR: Accord relatif au transport international des marchandises dangereuses par route (European Agreement Concerning the International
Carriage of Dangerous Goods by Road)
IMDG: International Maritime Code for Dangerous Goods
DOT: US Department of Transportation
IATA: International Air Transport Association
EINECS: European Inventory of Existing Commercial Chemical Substances
CAS: Chemical Abstracts Service (division of the American Chemical Society)
NFPA: National Fire Protection Association (USA)
HMIS: Hazardous Materials Identification System (USA)
VOC: Volatile Organic Compounds (USA, EU)
LC50: Lethal concentration, 50 percent
LD50: Lethal dose, 50 percent
PBT: Persistent, Bioaccumulative and Toxic
vPvB: very Persistent and very Bioaccumulative
NIOSH: National Institute for Occupational Safety
OSHA: Occupational Safety Health
TLV: Threshold Limit Value
PEL: Permissible Exposure Limit
REL: Recommended Exposure Limit
Acute toxicity - oral 4: Acute toxicity - Category 4
References
EMS SDS "Indigo Carmine, DcI-12" 6/05/2025 https://www.emsdiasum.com/docs/technical/msds/16910.pdf
Sigma-Addrich SDS "Indigo carmine" 4/11/2025 https://www.sigmaaldrich.com/AU/en/sds/SIAL/131164?userType=undefined
Sigma-Addrich SDS "Indigo carmine" 4/11/2025 https://www.sigmaaldrich.com/AU/en/sds/SIAL/131164?userType=undefined
This Safety Data Sheet (SDS) has been prepared in compliance with the Preparation of Safety Data Sheets for Hazardous Chemicals Code of Practice February 2016. It is the user's responsibility to determine the suitability of this information for adoption of necessary safety precautions. The information published in this SDS has been compiled from the publications listed in Section 16: to the best of our ability and knowledge these publications are considered accurate. We reserve the right to revise Safety Data Sheets as new information becomes available. Copies may be made for non-profit use.